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Rapidly differentiate Haemophilus species on the basis of the detection of porphyrin synthesis using Thermo Scientific™ Remel™ ALA Disk. In 1974, Kilian described a rapid method for identification of X-factor independent Haemophilus spp. based on the ability to synthesize heme precursors from ALA1. Lund and Blazevic determined the porphyrin test is more rapid and accurate for identification of X-factor-independent strains of Haemophilus (e.g. H. parainfluenzae and H. parahaemolyticus) than the satellite test2.
| Description | ALA Disk™ |
| Detectable Analytes | Porphyrin |
| Type | ALA Disk |
| Quantity | 25 Disks/Vial |
| Unit Size | Each |
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Members of the genus Haemophilus are small non-motile Gram-negative bacilli traditionally defined by a requirement for X and/or V factor3. X factor is derived from hemoglobin and V factor through the satellite relationship with Staphylococcus aureus.
Rapidly differentiate Haemophilus species on the basis of their detection of porphyrin synthesis using ALA Disk.
- Ready to use: Reagent-impregnated disk
- Rapid test: Results are obtained within a few hours.
- Accurate test: For detection of porphyrins which are only excreted by X-factor-independent Haemophilus spp.
- Easy to identify: A positive result showcases bright yellow fluorescence under long wave ultraviolet light.
After incubation the disk is exposed to long wave ultraviolet light in a dark room for any indication of fluorescence. Biberstein et al. studied the action of Haemophilus on δ-aminolevulinic acid (ALA) and reported a perfect correlation between X-factor-independence and the ability to convert ALA to porphyrins4.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Kilian, M. 1974. Acta Pathol. Microbiol. Scand. Sect B. 82:835-842.
- Lund, M.E. and D.J. Blazevic. 1977. J. Clin. Microbiol. 5:142-144.
- Winn, W., S. Allen, J. William, E. Koneman, G. Procop, P. Schreckenberger, and G. Woods. 2006. Koneman’s Color Atlas and Textbook of Diagnostic Microbiology. 6th Lippincott Williams and Wilkins, Baltimore, MD.
- Biberstein, E.L., P.D. Mini, and M.G. Gills. 1963. J. Bacteriol. 86:814-819.
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