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Presumptively identify anaerobic Gram-negative bacteria using Thermo Scientific™ Oxoid™ An-Ident Discs. Based on the differences in response to a number of antibiotics, Sutter and Finegold1 proposed a method for identification of Gram-negative anaerobic bacilli. Using carefully chosen levels of colistin, erythromycin, kanamycin, vancomycin penicillin and rifampicin with disc diffusion sensitivity testing techniques, they showed that these bacteria could be placed fairly consistently into five groups. Other simple biochemical tests and cultural characteristics were used for further identification.
Description | An-Ident Discs |
Detectable Analytes | Anaerobic Gram-negative bacteria |
Format | Disk, Cartridge |
Type | An-Ident Disk |
Packaging Type | 6 Cart./Pk. |
Quantity | 50 Discs/Cartridge |
Unit Size | Each |
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Use An-Ident Discs as a rapid and simple method for presumptive identification of anaerobic Gram-negative bacteria. They are primarily designed to allow easy identification of Bacteroids fragilis, the anaerobe species most commonly isolated in the routine hospital laboratory.
Rapid Results
Antibiotic levels on the discs are as follows:
Erythromycin (code E60) - 60μg
Rifampicin (code RD15) - 15μg
Colistin (code CT10) - 10μg
Penicillin (code P2) - 2 units
Kanamycin (code K1000) - 1000μg
Vancomycin (code VA5) - 5μg
An-Ident Discs are not suitable for antibiotic susceptibility testing to assess the clinical effectiveness of the various antibiotics.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.