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Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis, and Campylobacter jejuni using a reagent-impregnated Thermo Scientific™ Remel™ Hippurate Disk. Hwang and Ederer employed the ninhydrin reagent in a rapid method to detect hippurate hydrolysis by the end product glycine1. Jolly later outlined minimal criteria for identification of G. vaginalis including hippurate hydrolysis2. In 1989, Cacho et al. further evaluated the use of the hippurate disk in the identification of Campylobacter spp3.
| Description | Hippurate Disk |
| CE Marker | Yes |
| Format | Vial |
| Packaging Type | 1 Kit |
| Quantity | 25 Tests/Kit |
| Unit Size | Each |
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Rapidly detect the hydrolysis of sodium hippurate by β-hemolytic streptococci, Gardnerella vaginalis, and Campylobacter jejuni using a reagent-impregnated Hippurate Disk. The hippurate hydrolysis test determines the ability of Group B streptococci, as well as other bacteria, to enzymatically hydrolyze sodium hippurate4,5.
- Ready to use: Reagent-impregnated disk
- Rapid test: Results are obtained within 30 minutes.
- Easy to identify: A condensation reaction occurs with hydrindantin, ammonia, and residual ninhydrin to produce the final purple colored complex for a positive reaction and light gray color for a negative reaction.
Hippuric acid is hydrolyzed by the enzyme hippuricase to glycine and benzoic acid. Ninhydrin evokes a five-step reaction beginning with the deamination of glycine to form hydrindantin (a reduced form of ninhydrin), carbon dioxide, and ammonia.
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Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.
- Hwang, M. and G.M. Ederer. 1975. J. Clin. Microbiol. 1:114-115.
- Jolly, J.L.S. 1983. J. Clin. Pathol. 36:476-478.
- Cacho, J.B., P.M. Aguirre, A. Hernanz, and A.C. Velasco. 1989. J. Clin. Microbiol. 27:359-360.
- Greenwood, J.R. and M.J. Pickett. 1979. J. Clin. Microbiol. 9:200-204.
- MacFaddin, J.F. 2000. Biochemical Tests for Identification of Medical Bacteria. 3rd ed. Lippincott Williams & Wilkins, Philadelphia, PA.
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