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Presumptively identify Escherichia coli by fluorogenic assay procedure using Thermo Scientific™ Remel™ MUG Disk. In 1976, Kilian and Bulow reported the enzyme β-glucuronidase is present in most strains of E. coli (97%)1. The substrate, 4-methylumbelliferyl- β-D-glucuronide (MUG), is found to be both sensitive and selective for detection of β-glucuronidase activity. Trepeta and Edberg used the MUG test in conjunction with oxidase, indole, and lactose fermentation for rapid, cost effective identification of E. coli2.
Detectable Analytes | E. coli |
Type | E. coli Testing Kit |
Quantity | 25 Disks/Pk. |
Unit Size | Each |
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R21135 | Each | 25 Disks/Pk. | Request A Quote | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Godsey et al. supported their findings in an evaluation of methods based on microbial enzyme activity profiles3. Most strains of E. coli produce an enzyme β-D-glucuronidase. In the indole test, the enzyme tryptophanase attacks tryptophan molecule on its side chain, leaving the aromatic ring in the form of indole. The indole is then detected by addition of ρ-dimethylamino-benzaldehyde (Kavocs’ Reagent) which produces a red color. β-D-glucuronidase is an enzyme that cleaves the substrate 4-methylumbelliferyl-β-D-glucuronide (MUG) and thereby produces a fluorescent product called methylumbelliferone4. This compound is detectable using a long wave ultraviolet light (360 nm).
Interpretation:
MUG Disk Test (Direct or Tube Test):
Positive test - Blue fluorescence observed under 360 nm UV light
Negative test - No fluorescence observed under 360 nm UV light
Indole Test:
Positive test - Red color development on the disk or in the tube
Negative test - No red color development on the disk or in the tube