PathoProof Resources
FAQs
Analytical specificity and sensitivity of the PathoProof Mastitis PCR assay validated using culture isolates
(PDF) (J. Holopainen, et. al)
PathoProof Mastitis PCR Complete-12 assay is a real-time PCR based reagent kit for identification of all major mastitis-causing pathogens and the beta-lactamase gene responsible for penicillin resistance in staphylococci.
Real-time PCR test kit for rapid identification of
Staphylococcus aureus, Streptococcus agalactiae,
and
Mycoplasma bovis
from fresh or preserved bovine milk
(PDF) (M. Silvennoinen, et. al)
Identification of the contagious mastitis pathogens Staphylococcus aureus, Streptococcus agalactiae, and Mycoplasma bovis is important for the dairy industry and for mastitis management. It would be convenient and efficient if these microorganisms could be screened from DHI and/or bulk tank milk samples.
PathoProof Mastitis PCR asssay and sensitivity validation of a new test kit for rapid identification of
Staphylococcus aureus, Streptococcus agalactiae,
and
Mycoplasma bovis
from bovine milk
(PDF) (M. Silvennoinen, et. al)
PathoProof Mastitis Major-3 kit enable a four-hour detection of the contagious pathogens Mycoplasma bovis, Staphylococcus aureus, and Streptococcus agalactiae. The kit is very sensitive, and it can be used with preserved milk samples, providing tremendous possibilities for more efficient mastitis management.
Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing
(Full Abstract in New Window) (S. Taponen, et. al)
In more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 hours of conventional culture. The "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. This study provides the first investigation of the bacterial etiology of such samples, using a real-time PCR-based commercial reagent kit. The assay targets the DNA of the 11 most common bacterial species or groups in mastitis and the staphylococcal blaZ gene (responsibile for penicillin resistance) and ccan identify and quantify bacterial cells even if dead or growth-inhibited. A study was made of 79 mastitic milk samples with no-growth bacteria in convential culture, originating from cows with clinical mastitis.
Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identifican of bovine mastitis pathogens
(Full Abstract in New Window) (M.T. Koskinen, et. al)
Intramammary infection (IMI), also known as mastitis, is the most frequently occuring and economically the most important infectious disease in dairy cattle. This study provides a validation of the analytical specificity and sensitivity of a real-time PCR-based assay that identifies 11 major pathogen species or species groups responsible for IMI, and a gene coding for staphylococcal ß-lactamase producing production (penicillin resistance). Altogether, 643 culture isolates originating from clinical bovine mastitis, human, and companion animal samples were analyzed using the assay.